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戴冕鹤性别的分子鉴定
Molecular Sex Identi?cation of Grus nigricllis
于海龙, 张剑峰, 何宗亮, 贾晓旭, 杜文兴*
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作者单位:(南京农业大学动物科技学院, 南京 210095)
中文关键字:戴冕鹤;性别鉴定;非损伤性采样
英文关键字:Grus nigricllis; sex determination; non-invasive sampling
中文摘要:戴冕鹤Grus nigricllis为单态性鸟,很难通过外观和形态区分性别。本文采用非损伤性采样羽毛提取DNA,利用P2-P8引物对CHD基因进行特异性扩增,分别用HaeIIIAsp700I处理PCR产物,然后经过限制性片段长度多态性分析,HaeIII处理后的PCR产物,雄性出现2条带,雌性出现3条带;Asp700I处理后的PCR产物,雄性出现1条带,雌性出现3条带,表明可以准确鉴定戴冕鹤的性别。本文建立了从非损伤采样的羽毛提取DNA,利用PCR和限制性片段长度多态性准确鉴定戴冕鹤性别的方法,为人工繁殖戴冕鹤时合理配置雌雄比例、提高繁殖率奠定了基础。
英文摘要:Grus nigricllis is not a kind of sexually dimorphic bird, it’s difficult to identify the sex morphologically. In this paper, genomic DNA was extracted from feathers using a non-invasivesampling method. A PCR test was used employing P2-P8 primers that amplify the CHD gene specifically. The PCR productsof male and female appear as almost the same size band in electrophoresis analysis. Additionally, the PCR products were digested with HaeIII and Asp700I for RFLP analysis. Male PCR products showed two bands after digestion with HaeIII, while females showed three bands. Male PCR products showed a unique band after digestion with Asp700I, while females showed three bands. Results from these two tests can accurately identify the sex of G. nigricllis. In this paper, a method of accurately sexing G. nigricllis was established, while extraction of DNA from feathers using a non-invasivesampling method, a PCR test and RFLP analysis were used. This method laid a foundation for rational allocation of female-male proportions and improving reproduction ratios when artificial propagation of G. nigricllis is adopted.
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国内统一连续出版物号:51-1193/Q |国际标准出版物号:1000-7083
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