Beagle犬正常血液学指标及血清生化指标测定
Normal Blood Parameters and Serum Biochemical Indices in Beagles
李秋波，康纪平*，冯晓科，杨杰
点击：929次 下载：2次
DOI：
作者单位：(四川养麝研究所，四川都江堰 611830)
中文关键字：Beagle犬；血液学指标；血清生化指标
英文关键字：Beagle dogs; blood parameters; serum biochemical indices
中文摘要：选用普通级健康Beagle犬270只(雄性135只，雌性135只), 6～8月龄，体重7～9 kg，前肢内侧隐静脉采血，使用日本西森美康(Sysmex)全自动五分类血球仪及配套试剂和日本奥林巴斯(OLYMPUS—2700)全自动生化分析仪及配套试剂，分别测定血液学指标和血清生化指标。数据经统计分析，得出了雄性、雌性Beagle犬血液学指标和血清生化指标正常参考值的均值及标准差；19项血液学指标雌雄间差异不显著(P>0.05)；4项血液学指标：平均血小板体积(MPV)、血小板压积(PCT)、血小板体积分布宽度(PDW)、网织红细胞(Ret)与国内相关报道差异显著(P<0.05)；1项血清生化指标：碱性磷酸酶(ALP)雌雄间差异显著(P<0.05)，其余9项血清生化指标雌雄间差异不显著(P>0.05)。建立了本研究所繁育饲养基地Beagle犬血液学指标和血清生化指标的正常参考值范围，为医学研究提供参考基础数据。
英文摘要：In order to obtain baseline data on blood parameters and biochemical characteristics of beagle blood for experimental references, 270 dogs, aged 6~8 months and weighing 7~9 kg (135 female and 135 male), were measured with a Sysmex automatic hematology analyzer and OLYMPUS-2700 automatic chemistry analyzer. In 19 blood parameters there were no significant difference between females and males (P>0.05), while 4 hematological parameters including MPV (P<0.05), PCT (P<0.05), PDW (P<0.05) and Ret (P<0.05) had significant differences from other domestically reported results. Additionally, 1 serum biochemical parameter ALP (P<0.05) was significantly different between females and males. Nine serum biochemical indices had no significant difference between females and males (P>0.05). The data in this research could provide a reference for experiments involving beagles.