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环渤海红条毛肤石鳖种群遗传多样性研究
Genetic Diversity of Acanthochiton rubrolineatus in the Bohai Rim
王儒晓, 李媛媛, 刘传林, 杜文晓, 侯建海, 姚文君, 张俊凯, 张运发, 王宇, 曲江勇*
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DOI:10.11984/j.issn.1000-7083.20180208
作者单位:烟台大学生命科学学院, 山东烟台 264005
中文关键字:红条毛肤石鳖;生态遗传;遗传结构;细胞色素c氧化酶亚基Ⅰ
英文关键字:Acanthochiton rubrolineatus; ecological genetics; genetic structure; cytochrome c oxidase subunit Ⅰ
中文摘要:以线粒体细胞色素c氧化酶亚基Ⅰ(COⅠ)为遗传标记分析了环渤海红条毛肤石鳖Acanthochiton rubrolineatus 9个种群的遗传多样性及遗传结构。126只个体经PCR扩增测序获得654 bp的COⅠ基因序列,41个多态位点产生29种单倍型,单倍型多样性为0.899±0.013,核苷酸多样性为0.013 3±0.006 8。种群遗传多样性与纬度(r=-0.808,P<0.05)及年平均温度变异系数(r=-0.795,P<0.05)呈显著负相关,表明红条毛肤石鳖适应低纬度及温度稳定的海洋环境。分子方差分析表明,遗传变异主要发生在种群内(83.26%,P<0.001)。系统发生树与单倍型网络图没有呈现明显的谱系地理结构。种群历史动态结果显示,红条毛肤石鳖在早更新世晚期(第二温暖期)间冰期经历了种群扩张。
英文摘要:The genetic diversity and genetic structure of 9 populations of Acanthochiton rubrolineatus in the Bohai Rim were analyzed using mitochondrial cytochrome c oxidase subunit Ⅰ (COⅠ). The COⅠ gene (654 bp) of 126A. rubrolineatus individuals were amplified and sequenced. Finally, a total of 29 haplotypes were detected by 41 polymorphic loci. The haplotype diversity of all samples was 0.899±0.013, and the nucleotide diversity was 0.013 3±0.006 8. There was a significant negative correlation between the population genetic diversity and latitude (r=-0.808, P<0.05) and annual mean temperature variation coefficient (r=-0.795, P<0.05), indicating that A. rubrolineatus was adaptive in the marine environment with low latitudes and stable temperature. The result of analysis of molecular variance showed that genetic variation occurred mainly within populations (83.26%, P<0.001). Phylogenetic tree and haplotype network did not show obvious phylogeographic structure. Demographic results showed that A. rubrolineatus had experienced population expansion during the late period of early Pleistocene (the second warm period) and interglacial period.
2019,38(1): 20-27 收稿日期:2018-07-18
分类号:Q915.817;Q953
基金项目:国家自然科学基金地区基金项目(31460562);烟台市科技局科研计划项目(SM17SK04);烟台大学博士科研启动项目(SM15B01);山东省自然科学基金项目(ZR2107LC004);烟台大学大学生科技创新训练项目
作者简介:王儒晓(1995—),男,硕士研究生,研究方向:海洋生物学,E-mail:wrx_mamba@163.com
*通信作者:曲江勇,E-mail:qjy@ytu.edu.cn
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