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美洲大蠊精粉对小鼠血糖和肌酐的影响
Effects of Periplaneta americana Powder on Blood Glucose and Creatinine in Mice
高洁1, 刘瑾华1, 孙晓红1, 刘诗尧1, 沈咏梅2, 张修月1, 岳碧松1*
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DOI:10.11984/j.issn.1000-7083.20180203
作者单位:1. 四川大学生命科学学院, 生物资源与生态环境教育部重点实验室, 成都 610065;
2. 药用美洲大蠊四川省重点实验室, 成都 610031
中文关键字:美洲大蠊;高脂饮食;血糖;肌酐
英文关键字:Periplaneta americana; high-fat diet; blood glucose; creatinine
中文摘要:目的 研究美洲大蠊Periplaneta americana精粉对高脂饮食小鼠体质量、血糖和肌酐的影响。方法 高脂饮食小鼠分为对照组和实验组(给予美洲大蠊精粉:低剂量100 mg·kg-1、中剂量200 mg·kg-1、高剂量400 mg·kg-1)。灌胃第42天和第49天取血后;用试剂盒测定血清血糖和肌酐的含量;实时荧光定量PCR法检测肝脏中葡萄糖-6-磷酸酶(G-6-Pase)、葡萄糖转运蛋白2(GLUT2)和肾脏中单核细胞趋化蛋白-1(MCP-1)mRNA的表达水平。结果 与对照组相比,中、高剂量组体质量增长率在第42天和第49天均显著低于对照组(P<0.05,P<0.01)。第42天实验组小鼠的血糖、肌酐和MCP-1 mRNA水平与对照组相比有降低趋势,但差异无统计学意义(P>0.05),中、高剂量组的G-6-Pase mRNA水平极显著低于对照组(P<0.01),GLUT2 mRNA水平的差异无统计学意义(P>0.05)。第49天,低、高剂量组小鼠的血糖和肌酐水平显著低于对照组(P<0.05),且低、中、高剂量组的G-6-Pase、GLUT2和MCP-1 mRNA水平显著低于对照组(P<0.05,P<0.01)。结论 美洲大蠊精粉可降低高脂饮食小鼠的体质量增长率以及血糖、血清肌酐水平,减少肝脏中G-6-Pase mRNA表达和调节GLUT2 mRNA表达水平可能是美洲大蠊精粉降血糖机制之一,并且可能通过降低肾脏中MCP-1 mRNA的表达减轻肾脏损伤。
英文摘要:Objective To investigate the effects of Periplaneta americana powder on the body mass, blood glucose and creatinine in mice fed with high-fat diet.Methods The mice fed with high-fat diet were divided into control and experimental groups (P. americana powder low dose group 100 mg·kg-1; medium dose group 200 mg·kg-1 and high dose group 400 mg·kg-1); and the body mass changes were observed. The serum samples were taken and the fasting blood glucose and creatinine levels were then measured; the mRNA expression levels of glucose-6-phosphatase (G-6-Pase) and glucose transporter 2 (GLUT2) in liver tissues and monocyte chemoattractant protein-1 (MCP-1) in kidney tissues were detected by real-time PCR at 42nd and 49th day.Results The body mass growth rates of the middle and high dose groups declined significantly after 42 and 49 days compared to the control (P<0.05, P<0.01). On the 42nd day, the content of blood glucose, creatinine and MCP-1 mRNA levels in mice of experimental groups showed the decreasing trends, but these differences were not significant (P>0.05); G-6-Pase mRNA levels in the middle and high dose groups were dramatically lower (P<0.01); GLUT2 mRNA levels did not change notably (P> 0.05). On the 49th day, the content of blood glucose and creatinine in the low and high dose groups were significantly lower than those of the control (P<0.05); G-6-Pase, GLUT2 and MCP-1 mRNA levels in the low, medium and high dose groups were remarkably decreased (P<0.05, P<0.01).Conclusion P. americana powder can reduce the body mass growth rate, the content of blood glucose and creatinine in mice fed with high-fat diet. Decreasing the expression of G-6-Pase mRNA and regulating the expression of GLUT2 mRNA in liver may be one of the mechanisms of P. americana powder to decrease the blood glucose level. Moreover, P. americana powder may reduce the renal injury by declining the expression of MCP-1 mRNA in kidney.
2018,37(6): 667-671 收稿日期:2018-07-09
分类号:Q95-33
作者简介:高洁,女,硕士研究生,研究方向:动物分子生物学
*通信作者:岳碧松,E-mail:bsyue@scu.edu.cn
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