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楔形两极虫(黏体门,双壳目)的形态学重描述及其近缘种的分子系统学研究
Redescription of Myxidium cuneiforme Fujita, 1924 (Myxosporea: Bivalvulida) and Molecular Phylogeny with its Relative Species
李超, 索栋, 杨承忠*, 赵元莙
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作者单位:重庆师范大学生命科学学院, 动物生物学重庆市重点实验室, 重庆 401331
中文关键字:楔形两极虫;近缘种;18S rDNA;分子系统学
英文关键字:Myxidium cuneiforme; relative species; 18S rDNA; molecular phylogeny
中文摘要:两极虫属Myxidium和楚克拉虫属Zschokklella在形态上非常相似,形态鉴定界限模糊。为进一步厘清两者的分类学关系,本研究对采自涪江重庆市潼南县江段的楔形两极虫M. cuneiforme Fujita,1924进行了形态学重描述,并对其分子系统学进行了研究。楔形两极虫孢子壳面观呈长条形,中部稍凹陷或孢子一边突出,壳瓣上有6~8条与缝嵴平行的条纹;缝嵴直,缝面观呈梭形。孢子长12.5 μm±0.3 μm,宽5.4 μm±0.3 μm(n=20)。极囊2个,呈梨形,分布于孢子两极端;极囊长4.3 μm±0.2 μm,宽3.1 μm±0.2 μm(n=20),极丝细长且明显,盘曲5~6圈。以18S rDNA为分子标记,对楔形两极虫及其近缘种进行了保守区变异、遗传距离、序列相似度和分子系统发育分析,结果表明:两极虫属和楚克拉虫属均非单系发生,两属间物种相互交叉聚支;两极虫属与楚克拉虫属物种间具有很近的亲缘关系。本文基于形态和分子数据的研究结果支持将两极虫属和楚克拉虫属合并为一个属的观点。
英文摘要:The species identification between Myxidium and Zschokklella is difficult due to their similar morphology and vague boundaries. To clarify the taxonomic relationship of the two genera, the morphology of Myxidium cuneiforme Fujita, 1924 collected from the Fujiang River in Tongnan county, Chongqing, China was redescribed. Subsequently, molecular phylogeny was analyzed along with the relative species. The mature spore of M. cuneiforme was elongated in valvular view with the middle part concaved slightly. The surface of each valve had 6~8 longitudinal grooves. The suture line was straight, and spore was fusiform in sutural view. The spore was 12.5 μm±0.3 μm long and 5.4 μm±0.3 μm wide (n=20), each with two polar capsules posited at the both end of the spore. The two polar capsules were pyriform with equal size of 4.3 μm±0.2 μm long and 3.1 μm±0.2 μm wide (n=20), containing polar filaments coiled 5~6 turns. Analyses of the variation of conserved regions, genetic distances, sequence similarities and molecular phylogeny based on 18S rDNA sequences indicated that, Myxidium and Zschokkella were not monophyletic, as they clustered together with each other in the phylogenetic analysis. Therefore, Myxidium and Zschokkella had a very close genetic relationship. The results from the morphological and molecular data supported the proposal that Myxidium and Zschokkella should be assigned to one single genus.
2016,(35): 384-390 收稿日期:2015-11-06
DOI:10.11984/j.issn.1000-7083.20150350
分类号:Q959.115
基金项目:国家自然科学基金项目(No.31471980,No.31501845);重庆市科委基金项目(No.cstc2014jcyjA80014);重庆市教委科技项目(KJ1400502)
作者简介:李超(1989-),男,硕士研究生,研究方向为寄生虫进化生态学,E-mail:1027276898@qq.com
*通讯作者:杨承忠,E-mail:drczyang@126.com
参考文献:
陈启鎏, 马成伦. 1998. 中国动物志 粘体门 粘孢子纲(淡水)[M]. 北京:科学出版社:1-30.
鲁义善, 聂品. 2004. 淡水鱼类粘孢子虫18S rDNA分子系统学研究[J]. 水生生物学报, 28(6):587-591.
冉佼, 杨承忠, 赵元莙. 2014. 基于遗传距离的粘孢子虫分类研究[J]. 重庆师范大学学报(自然科学版), 31(3):31-34.
宋微波, 赵元莙, 徐奎栋, 等. 2003. 海水养殖中的危害性原生动物[M]. 北京:科学出版社:315-428.
张婧, 莫西, 李楠楠, 等. 2015a. 徐家汇尾孢虫Henneguya zikawiensis Sikama, 1938补充描述及其分子系统学研究[J]. 内江师范学院学报, 30(4):42-46.
张婧, 杨承忠, 赵元莙. 2015b. 碘泡科(粘体门:粘孢子纲:双壳目)动物的系统分类学研究[J]. 四川动物, 34(3):474-480.
赵元莙, 马成伦, 宋微波. 2001. 海水养殖中原生动物病原虫的鉴定-Ⅱ 粘孢子虫原生动物的研究及鉴定方法[J]. 青岛海洋大学学报(自然科学版), 31(5):681-688.
钟霞, 毛华明, 邓卫东. 2007. 18S rRNA/rDNA序列分析技术在瘤胃原虫分类学研究中的应用[J]. 中国畜牧兽医, 34(12):56-58.
Barta JR, Martin DS, Libertor PA, et al. 1997. Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences[J]. The Journal of Parasitology, 83(2):262-271.
Bartošová P, Fiala I, Hypša V. 2009. Concatenated SSU and LSU rDNA data confirm the main evolutionary trends within myxosporeans (Myxozoa:Myxosporea) and provide an effective tool for their molecular phylogenetics[J]. Molecular Phylogenetics and Evolution, 53(1):81-93.
Canning EU, Okamura B. 2004. Biodiversity and evolution of the myxozoa[J]. Advances in Parasitology, 56:44-131.
Diamant A, Lom J, Dyková I. 1994. Myxidium leei n. sp., a pathogenic myxosporean of cultured sea bream Sparus aurata[J]. Diseases of Aquatic Organisms, 20(2):137-141.
Dyková I, Tyml T, Fiala I, et al. 2007. New data on Soricimyxum fegati (Myxozoa) including analysis of its phylogenetic position inferred from the SSU rRNA gene sequence[J]. Folia Parasitologica, 54(4):272-276.
Eiras JC, Saraiva A, Santos MJ, et al. 2011. Synopsis of the species of Myxidium Bütschli, 1882 (Myxozoa:Myxosporea:Bivalvulida)[J]. Systematics Parasitology, 80:81-116.
Fiala I. 2006. The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis[J]. International Journal for Parasitology, 36(14):1521-1534.
Fujita T. 1924. Studies on myxosporidian infection of the crucian carp[J]. Japanese Journal of Zoology, 1:45-57.
Kent ML, Andree KB, Bartholomew JL, et al. 2001. Recent advances in our knowledge of the Myxozoa[J]. The Journal of General and Applied Microbiology, 48(4):395-413.
Lom J, Dyková I. 2006. Myxozoan genera:definition and notes on taxonomy, life cycle terminology and pathogenic species[J]. Folia Parasitologica, 53:1-36.
Michel R, Walochnik J, Aspöck H. 2003. Pseudodidymium cryptomastigophorum gen. n., sp. n., a Hyperamoeba or a slime mould? A combined study on morphology and 18S rDNA sequence data[J]. Acta Protozoologica, 42(4):331-342.
Michielsen NC. 1966. Intraspecific and interspecific competition in the shrews Sorex araneus L. and S. minutus L.[J]. Archives Neerlandaises de Zoologie, 17(1):73-174.
Prunescu CC, Prunescu P, Pucek Z, et al. 2007. The first finding of myxosporean development from plasmodia to spores in terrestrial mammals:Soricimyxum fegati gen. et sp. n. (Myxozoa) from Sorex araneus (Soricomorpha)[J]. Folia Parasitologica, 54:159-164.
Ronquist F, Huelsenbeck JP. 2003. MrBayes 3:Bayesian phylogenetic inference under mixed models[J]. Bioinformatics, 9:1572-1574.
Swofford DL. 2001. PAUP:phylogenetic analysis using Parsimony (and other methods) version 4[M]. Sunderland, Massachusetts:Sinauer Associates.
Tamura K, Stecher G, Peterson D, et al. 2013. MEGA6:molecular evolutionary genetics analysis version 6.0[J]. Molecular Biology and Evolution, 30:2725-2729.
Whipps CM, Zhao YJ. 2015. Synopsis of the species of the genus Sphaeromyxa Thélohan, 1892 (Myxosporea:Bivalvulida:Variisporina Sphaeromyxidae[J]. Systematics Parasitology, 92(2):81-99.
Wright ADG, Lynn DH. 1997. Phylogenetic analysis of the rumen ciliate family Ophryoscolecidae based on 18S ribosomal RNA sequences, with new sequences from Diplodinium, Eudiplodinium, and Ophryoscolex[J]. Canadian Journal of Zoology, 75(6):963-970.
Zhao YJ, Li NN, Tang FH, et al. 2013. Remarks on the validity of Myxobolus ampullicapsulatus and Myxobolus honghuensis (Myxozoa:Myxosporea) based on SSU rDNA sequences[J]. Parasitology Research, 112(11):3817-3823.
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