英文摘要:Brine shrimp
Artemia is an important model organism and economic animal resource. The microsatellite loci of this species have a significant research and application value. In this study, the
Artemia microsatellite enriched DNA libraries of (AC)
n and (AG)
n were conducted successfully using the method of FIASCO (fast isolation by AFLP sequence containing repeats). In the (AC)
n library, 67 colonies (total no. 120) were picked out and sequenced, with a positive clone rate of 55.8%. In the (AG)
n library, 69 colonies out of 200 colonies were sequenced, with a positive clone rate of 34.5%. Among all the sequences containing microsatellites, 63 pairs of primers were designed and synthesized for further primer screening. By using 18
Artemia DNA samples from Qinghai and Tibetan, 6 pairs of primers with polymorphic microsatellite loci of
Artemia were identified. These polymorphic loci may facilitate further study on the detection of genetic diversity, genetic linkage mapping and the location of quantitative traits of
Artemia.
2016,(35): 356-360 收稿日期:2015-10-13
DOI:10.11984/j.issn.1000-7083.20150317
分类号:Q959.223;Q78
基金项目:青海省应用基础研究计划项目(2013-Z-750;2013-Z-751)
作者简介:刘海珍(1989-),女,硕士研究生
*通讯作者:马建滨,E-mail:mjb_117@163.com;都玉蓉,xndyr@163.com
参考文献:
都玉蓉, 马建滨, 徐永涛, 等. 2014. 藏羚羊(AC)n,(AG)n微卫星文库构建[J]. 四川动物, 33(1):19-22.
李强, 万建民. 2005. SSRHunter, 一个本地化的SSR位点搜索软件的开发[J]. 遗传, 27(5):808-810.
马志珍, 陈汇远, 武振彬, 等. 1996. 中国内陆盐湖卤虫资源开发利用研究[J]. 海洋水产研究, 17(1):1-14.
马志珍. 1993. 中国卤虫生物地理分布的研究[J]. 现代渔业信息, 8(9):19-26.
印象初, 印红, 周可新, 等. 2001. 高原盐湖盐藻和卤虫资源的开发和利用[J]. 盐湖研究, 9(1):4-8.
袁慧, 张修月, 宋昭彬, 等. 2008. 岩原鲤微卫星富集文库的构建及微卫星分子标记[J]. 四川动物, 27(2):210-215.
郑绵平. 1995. 论"盐湖农业"[J]. 地球科学, 4:404-418.
郑绵平. 2001. 青藏高原盐湖资源研究的新进展[J]. 地球科学, 22:97-102.
Carleton KL, Streelman JT, Lee BY, et al. 2002. Rapid isolation of CA microsatellites from the tilapia genome[J]. Animal Genetics, 33(2):140-144.
Sambrook JW, Russell D. 2001. Molecular cloning:a laboratory manual, 3rd ed[M]. New York:Cold Spring Harbor Laboratory Press:463-470.
Weber JL. 1990. Informativeness of human (dC-dA)n·(dG-dT)n polymorphisms[J]. Genomics, 7(4):524-530.
Zane L, Bargelloni L, Patarnello T. 2002. Strategies for microsatellite isolation:a review[J]. Molecular Ecology, 11(1):1-16.