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基于粪便DNA的岩羊微卫星引物的筛选及个体识别
Microsatellite Primers Screening and Individual Identification of Blue Sheep Based on the Fecal DNA
刘旭1,2, 刘振生3,4, 丁由中1*, 王小明1
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作者单位:1. 华东师范大学生命科学学院, 上海 200062;
2. 国家海洋局海岛研究中心, 福建平潭 350400;
3. 东北林业大学野生动物资源学院, 哈尔滨 150040;
4. 国家林业局野生动物保护生物学重点实验室, 哈尔滨 150040
中文关键字:岩羊;微卫星;粪便DNA;个体识别;引物筛选
英文关键字:Pseudois nayaur; microsatellite; fecal DNA; individual identification; primers screening
中文摘要:贺兰山保护区岩羊Pseudois nayaur种群数量近年来增长很快,为了解贺兰山保护区的岩羊种群的遗传健康状况,从而更好地保护和管理岩羊种群,需要对岩羊种群的遗传多样性进行研究。在其近缘物种中选出36对微卫星引物对收集到的岩羊粪便DNA样品进行PCR扩增。结果发现,有9对引物在岩羊粪便DNA中扩增出了多态性位点。各位点基因杂合度介于0.26~0.95之间,平均杂合度为0.48,各位点多态信息含量介于0.23~0.68之间,平均多态性为0.48。
英文摘要:The blue sheep (Pseudois nayaur) population is growing rapidly in recent years in the Helan Mountain Nature Reserve. In this study, the genetic diversity of the blue sheep was investigated to assess the genetic status of this population for better protection and management. A total of 36 pairs of microsatellite primers were selected from the related species of blue sheep. The PCR amplification was carried out using the fecal DNA samples. The results showed that there were 9 loci polymorphisms in the blue sheep fecal DNA samples. The heterozygosity ranged from 0.26 to 0.95 with an average of 0.48. The polymorphism information content was between 0.23 and 0.68, and the average polymorphism content was 0.48.
2015,34(2): 169-173 收稿日期:2014-11-11
DOI:10.3969/j.issn.1000-7083.2015.02.002
分类号:Q959.8;Q78
基金项目:中央高校基本科研业务费资助项目(DL13EA01,2572014CA03)
作者简介:刘旭,博士,研究方向:保护生物学,E-mail:lxu0708@hotmail.com
*通讯作者:丁由中,讲师,研究方向:生态学,E-mail:yzding@kj.ecnu.edu.cn
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